Figure 6.

Administration of αPD-1 can significantly diminish worm burden in Rag1^−/−recipients. Rag1^−/− mice were treated with isotype or αPD-1 antibody (250 µg/mice), followed by rmIL-33 (200 ng/mice), for 3 d. Gating strategy included Lin⁻CD45⁺ Thy1⁺CD127⁺CD25⁺ST2⁺KLRG1⁺. Representative flow plots of ILC-2 in Isotype and αPD-1 cohorts (A) and summary of absolute numbers of KLRG1⁺ ILC-2 in Isotype and αPD-1 cohorts’ lungs (B) were evaluated. Rag1^−/− mice were infected with 300 Larvae 3 of N. brasiliensis on day 0. Anti–mouse PD-1 antibodies or IgG isotype control were i.p. injected into mice on day 0, 3, 6, and 9, at a dose of 250 µg antibody per mouse each time. Leukocytes were isolated from MLNs on day 14, and the numbers of total ILC-2 cells were analyzed by flow cytometry (C). Cytokine expression was analyzed using intracellular flow cytometry (D and E). Feces were collected on day 7, 8, and 9 from individual mouse, and the worm eggs were counted (F). Adult worms in small intestine were counted on day 14 (G). Animals per cohort was n = 4–5. Data shown are mean ± SEM. Experiments were repeated twice. Data on n = 5 mice is shown (A–C). A Student’s t test was performed to determine statistical significance for the data shown in all the panels. P ≤ 0.05 was considered significant. Significant p-values are denoted in the figures.