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. 2017 Jun 5;216(6):1623–1639. doi: 10.1083/jcb.201607096

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© 2017 Auckland et al.

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Figure 9. — Ska maturation correlates with loss of Bub1 from kinetochores. (a) Images of prometaphase HeLa cells stained with antibodies against Ska1 and Bub1 and CREST antisera. Zoom boxes depict the staining intensities at aligned bioriented (1 and 3), unaligned bioriented (2), and unaligned nonbioriented (4) kinetochore pairs. Bars: (zoom-out) 5 µm; (zoom-in) 1 µm. (b) Quantification of CREST-based intersister distance at the kinetochore pair subgroups shown in a. n = 159 KT from 16 cells. (c) Quantification of Ska1 staining intensity at the kinetochore pair subgroups defined in a. n = 159 KT from 16 cells. (d) Quantification of Bub1 staining intensity at the kinetochore pair subgroups defined in a. n = 159 KT from 16 cells. (e) Images of prometaphase cells stained with antibodies against Ska1 and Bub1 and CREST antisera. Zoom boxes depict an unaligned bioriented kinetochore pair with opposing Ska1/Bub1 loading. Bars: (left) 5 µm; (right) 1 µm.