Fig. 5.

Expression level of defense genes in pepper on different hours after treatments of water extract from spent mushroom substrate (WESMS) of Lentinula edodes. L. edodes WESMS, DL-β-amino butyric acid (BABA, positive control) and sterile distilled water (negative control) was treated on pepper roots and leaves and different hours (0, 48, 96, and 120 h) were given after the treatment. Total RNA was extracted from leaf samples of the treated peppers and subjected to RT-PCR using primers targeting CaPR4, CaPR10 and 18S rDNA (positive control). The PCR products were visualized on agarose gel (1.5%) by staining with ethidium bromide. M, 100-bp DNA ladder.