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Comparison of detection sensitivity between multiplex PCR and uniplex PCR for each of Citrus mosaic sadwavirus (CiMV), Citrus tristeza virus (CTV), and Citrus tatter leaf virus (CTLV). (A) Multiplex PCR. (B–D) Uniplex PCR for each of CiMV, CTLV, and CTV, respectively. Lane M: 100-bp DNA ladder; lanes 1–5: 10, 50, 100, 500, and 1,000 times dilution of cDNA from CSM-1 isolate.
