Table 2.

Primers used to detect the 4 citrus viruses in uniplex or multiplex PCR

Target (virus)	Primer		Amplified gene	Product size (bp)	Reference
	Name	Sequence (5′-3′)
CTV	CTCpo1	5′-TACCGTCCCCAAACCAACTA-3′	CP	738	Sekiya et al. (1991)
	CTCpo2	5′-CATGGCAGGTTATACAGTAC-3′
	CTV-52	5′-CGAGGTATCATTCTTCGAGC-3′	CP	640	Kano et al. (1998)
	CTV-32	5′-CGCCATAACTCAAGTTGCG-3′
	CTV-Po-F	5′-GTGGCCAATAGGTCCGTAGA-3′	CP	412	In this study
	CTV-Po-R	5′-CGGGTCTCAACCTAGCCATA-3′
CiMV	CISD-5	5′-ACCTGCGATATATTTGCAGG-3′	PP2	1,050	In this study
	CISD-3	5′-CAGAAATCAAGAAAAACACGC-3′CiMV
CiMV	Sadwa(F)	5′-ACGTTCTTTCCAAGGGGAGT-3′	PP2	818	In this study
	Sadwa(R)	5′-CTCCATCAAGGGAGTTTGGA-3′
SDV	SRNA1M	5′-YYRSAAATCAAGWAAAACAC-3′	S-58 RNA1	1,176	Ito et al. (2007)
	SRNA1P	5′-TYTKAARTGGAGYTGGATSGG-3′SDV
SDV	SDV(2014)-F	5′-CAACACATCGGGAGGAAACT-3′	PP2	745	In this study
	SDV(2014)-R	5′-AGCATGGAAGATGGACCTTG-3′
	SDV-CM	5′-CAGAAATCAAGAAAAACAC-3′	S-58 RNA2	1,526	Ito et al. (2007)
	SDV-CP	5′-GGGAAAGCTGCTTATTTAGG-3′
SDV/CiMV	FW146	5′-ACTAGGGATAGCGCCCTAG-3′	RNA2	342	Iwanami (2010)
	RV488	5′-GGACCGATATTGGGCCAT-3′
	PP2-1(F)	5′-ACATTCGGGCAGCAGCAAGAT-3′	PP2	883	In this study
	PP2-1(R)	5′-TCCYTGAGWGAGAGACCGTGC-3′
CTLV	ASGV-dn	5′-CTCGAGACCCTCCAGTTCCAAGTTA-3′	CP	720	Shim et al. (2004)
	ASGV-up	5′-CATATGAGTTTGGAAGACGTGCTTC-3′
	CTLV(2013)-F	5′-CGAAAACCCCTTTTTGTCCT-3′	CP	607	In this study
	CTLV(2013)-R	5′-ATAGACCCGGCAAAGGAACT-3′
Actin	Actin-F	5′-TCCACCATGTTCCCAGGTAT-3′	Actin	210	In this study
	Actin-R	5′-CATCTCTGTCTGCCACCTGA-3′

CTV, Citrus tristeza virus; CiMV, Citrus mosaic sadwavirus; SDV, Satsuma dwarf virus; CTLV, Citrus tatter leaf virus; CP, coat protein.