Figure 3.

Effect of immunosuppressive drugs (ISDs) on CD4⁺CD25^– cell ability to proliferate. CD4⁺CD25^– cell proliferation in the absence and presence of ISDs and before and after regulatory T cell (T_reg) addition was assessed by carboxyfluorescein succinimidyl ester (CFSE) staining and expressed as percentage of proliferating cells. (a) CFSE staining of CD4⁺CD25^– cells alone or in the presence of T_regs in one representative healthy subject (HS) and one autoimmune hepatitis (AIH) patient. Mean (± standard error of the mean) % proliferating CD4⁺CD25^– T cells in the presence of ‘no treatment’ (NT) or individual drugs in HS (n = 8) and AIH patients (n = 6) before (b) and after (c) T_reg addition.