Figure 3.

Anti‐angiogenic activity of Dolichos lablab lectin‐conditioned medium (DLL‐CM) in in‐vitro angiogenic assay models. Indirect angioinhibitory effect of DLL on various angiogenic systems in vitro. DLL‐CM was the supernatant of DLL exposed to murine splenocytes after 24 h incubation. DLL‐CM exhibits regressed angiogenesis in (a) ex‐vivo chorioallontoic membrane (CAM) and (b) rat aortic angiogenesis assay. DLL directly exhibited only a minimal angiolytic effect. (c) DLL‐CM exposed to human umbilical vein endothelial cells (HUVEC) cells inhibits the tube‐forming efficiency. Pictographical representation of (d) ex‐vivo CAM, (e) aortic ring angiogenesis and (f) tube formation assay. Data were represented as mean ± standard error of the mean (s.e.m.) of three independent experiments. Statistically significant values are expressed as *P < 0·05 and **P < 0·01. [Colour figure can be viewed at wileyonlinelibrary.com].