Figure 6.

Translational down‐regulation of tumoral angiogenic gene expression by Dolichos lablab lectin (DLL). Immunoblots, gelatin zymography and immunohistochemistry (IHC) were carried out using in‐vivo tumour cells of both Dalton's ascites lymphoma (DLA) and Dalton's lymphoma solid tumour (DLS) with or without DLL treatment in two different concentrations. (a,b) Immunoblots showing altered translational expression of proangiogenic genes such as nuclear factor kappa B (NF‐κB), inhibitory kappa B (I‐κB), hypoxia inducible factor 1α (HIF‐1α), vascular endothelial growth factor (VEGF)‐A, matrix metalloproteinase (MMP)‐2 and 9 in ascitic (panel 1) and solid tumour (panel 2). The graphs indicate the densitometric values of corresponding Western blot data. (c) Reduction of secreted serum VEGF levels in DLL‐treated DLA mice in vivo. (d) Gelatin zymography showing reduced gelatinolytic activity in DLL‐treated mice. (e) DLL‐conditioned medium (DLL‐CM) inhibits the migration of A549 cell in vitro. IHV should be replaced with IHC detection of DLL‐induced altered gene expression. (f,i) IHC detection of proangiogenic gene expression in solid tumours in vivo after DLL treatment. The intense brown staining indicates the respective protein expression. Representative graphs demonstrating the gene expression corresponding to the IHC data are given below. (j) Representative counter haematoxylin‐stained section. Data are represented as mean ± standard error of the mean (s.e.m.) of three independent experiments. Statistically significant values are expressed as *P < 0·05 and **P < 0·01. [Colour figure can be viewed at wileyonlinelibrary.com].