Figure 3. Perturbing GTP binding by Cdc10 disrupts the order of interactions with Cdc3.

Left, ribbon diagram of Sept2-GppNHp (PDB 3FTQ) models the location of the mutated Asp residue (red spheres) and bound nucleotide (orange). Center, violin plot of CSD-BiFC results, as in Figure 2C but with Cdc10(D182N)-V_C (‘10*-V_C’, ‘Cdc10*'). Right, cartoon of inferred order of assembly. From left to right, n = 60, 51, 95, and 62. Strains used were: 10(D182N)-V_C/3-V_N, 10(D182N)/10-C_N, and 10(D182N)-V_C/3-V_N/10C_N.

DOI: http://dx.doi.org/10.7554/eLife.23689.010