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Quantification of ciliated cells in amiRNA knockdown strains. The % ciliated cells in asynchronous cultures was determined by microscopic examination following formaldehyde fixation (n > 100 for all samples). All amiRNA strains had reduced numbers of ciliated cells.
| Strain |
% Ciliated cells
(n > 100) |
|---|---|
| EV3 | 58% |
| amiRNA1 #5 | 24% |
| amiRNA1 #6 | 8.7% |
| amiRNA2 #8 | 0% |
