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. Author manuscript; available in PMC: 2018 Jan 1.
Published in final edited form as: Semin Immunopathol. 2016 Nov 28;39(1):21–28. doi: 10.1007/s00281-016-0612-y

Fig. 2.

Fig. 2

Mouse naïve CD4+ T cells were cultured in Th9-polarizing conditions (TGFβ + IL-4) in absence or presence of the indicated TNF family cytokines on coated plate with anti-CD3 and anti-CD28 in absence (a) or in presence of APCs (b) and were analyzed for their IL-9 expression by flow cytometry after restimulation with PMA and ionomycin for 4 hrs.