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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Plant J. 2017 Apr 29;90(6):1196–1207. doi: 10.1111/tpj.13529

Figure 3. In vitro kinase assay demonstrating the phosphorylation of LYK3 by NORK.

Figure 3

Auto phosphorylation and transphosphorylation activity of LYK3 tagged with GST(indicated by phosphorylation of casein) along with the lack of kinase activity for the mutant version (G334E) of LYK3 (A), autophosphorylation and transphosphorylation activity of NORK tagged with MBP (indicated by phosphorylation of casein) along with the lack of kinase activity for the mutant version (G794E) of NORK (B), lack of phosphorylation of NORK by LYK3 indicated by absence of band corresponding to the size of mutant NORK and a band corresponding to the size of LYK3 in a LYK3 + mutant NORK reaction mix, phosphorylation of LYK3 by NORK indicated by presence of band corresponding to the size of mutant LYK3 and a band corresponding to the size of NORK in a mutant LYK3 + NORK reaction mix, no non-specific phosphorylation indicated by a band corresponding to LYK3 alone in a LYK3 + purified MBP alone reaction mix or a band corresponding to NORK alone in a NORK + purified GST reaction mix (C).