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. Author manuscript; available in PMC: 2018 Jun 1.

Published in final edited form as: J Allergy Clin Immunol. 2017 Feb 10;139(6):1885–1896.e9. doi: 10.1016/j.jaci.2016.12.971

FIG. 4 — Innate cell activation in peripheral blood measured by CyTOF. Peripheral blood was obtained before and after food challenge in 4 subjects, 2 with active FPIES and 2 with outgrown FPIES. Shown are SPADE plots indicating fold change (postchallenge vs prechallenge) in CD163 (A), CD25 (B), CD69 (C), and CD16 (D) expression across immune subsets. The size of the bubbles represents the cell number in each node, while the color intensity indicates fold change in the expression of the respective marker relative to prechallenge baseline. The cell populations are labeled as identified by pattern of surface marker expression. Note the increased expression of CD163 in the monocyte populations, and increased CD25 in CD56^dim NK cells, neutrophils, and eosinophils in subjects with FPIES, but not in outgrown subjects. CD69 expression shows upregulation across multiple immune compartments including the T-lymphocyte populations. SPADE, Spanning-tree progression analysis of density-normalized events.

FIG. 4 — Innate cell activation in peripheral blood measured by CyTOF. Peripheral blood was obtained before and after food challenge in 4 subjects, 2 with active FPIES and 2 with outgrown FPIES. Shown are SPADE plots indicating fold change (postchallenge vs prechallenge) in CD163 (A), CD25 (B), CD69 (C), and CD16 (D) expression across immune subsets. The size of the bubbles represents the cell number in each node, while the color intensity indicates fold change in the expression of the respective marker relative to prechallenge baseline. The cell populations are labeled as identified by pattern of surface marker expression. Note the increased expression of CD163 in the monocyte populations, and increased CD25 in CD56^dim NK cells, neutrophils, and eosinophils in subjects with FPIES, but not in outgrown subjects. CD69 expression shows upregulation across multiple immune compartments including the T-lymphocyte populations. SPADE, Spanning-tree progression analysis of density-normalized events.