Fig. 4.

27-OH suppresses lipogenesis and adipogenesis of 3T3-L1 cells. The cells were treated with DMSO (the vehicle control), 1 μM TO, 7 μM 27-OH, and 1 μM TO + 7 μM 27-OH for 10 days after induction of differentiation. Each gene expression level (LXRα, PPARγ, FAS, and aP2) was normalized to the β-actin level. Each gene expression of the vehicle control was arbitrarily set at 1. Data are presented as the mean ± SEM (n = 5). Columns marked with different letters are significantly different at P < 0.05. aP2, adipocyte fatty acid-binding protein; DMSO, dimethyl sulfoxide; FAS, fatty acid synthase; LXRα, liver X receptor alpha; 27-OH, 27-hydroxycholesterol; PPARγ, peroxisome proliferator-activated receptor gamma; TAG, triacylglycerol; TO, TO901317