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Effect of agitation rate on aggregation of microcarriers and growth of hMSCs on microcarriers. Two days after the inoculation of cells to microcarriers, the agitation rates were set to 30 (triangles), 60 (squares), and 90 (circles) rpm. Cell concentration (a) and the percentage of aggregated microcarriers (b) were determined. The average ±SD of triplicate cultures is shown
