Table 2.
Expressions of cell surface antigens on cells grown on microcarriers
| Percentage of antigen-positive cells (%) | ||||
|---|---|---|---|---|
| PDL | CD90 | CD166 | CD90 and CD166 | |
| Pre culture | ||||
| Dish | 4.0 | 74.3 | 92.5 | 67.4 |
| Main culture | ||||
| Dish | 7.6 | 58.1 | 96.6 | 65.0 |
| Microcarrier | 6.0 | 91.5 | 87.6 | 85.5 |
Cells grown on dishes (pre-culture) and harvested by trypsinization were inoculated to main cultures on microcarriers and dishes. These three types of cells harvested from the pre-culture (dishes) and main cultures on dishes and microcarriers were analyzed using flow cytometry