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. 2017 Jun 7;7:228. doi: 10.3389/fcimb.2017.00228

Figure 1.

Figure 1

E. chaffeensis Etf-1 PNA bound to the target ssRNA. (A) Etf-1 PNA targeted the putative Shine-Dalgarno region and translational start site (underline). (B) Dot blot analysis of biotin-labeled Etf-1 and streptavidin-HRP-labeled CTL PNA. Dot 1–4: Biotin-CTL PNA, Unlabeled CTL PNA, Unlabeled Etf-1 PNA, and Biotin-Etf-1 PNA. (C) Electrophoresis migration shift assay of biotinylated Etf-1 PNA bound to ssRNA. Lane 1, no labeled PNA, lanes 2–4 labeled PNA incubated with 0, 10 ×, and 1 × non-labeled PNA, respectively.