Figure 5.

Antigen-specific regulatory T cells (asTregs) control naïve T cell priming in hilar lymph node (hLN). (A) Experimental scheme for analyzing naïve T cell priming. DO11.10 spleen CD4⁺ T cells were used to test T cell priming. Asthmatic recipients were transferred asTregs for 7 days, then i.v. infused with donor DO11.10 CD4⁺ T cells. Maturation and polarization of these cells were analyzed within hLN using KJ1-26 as marker. Both antigen-matched (Co-OVA, red) and mismatched (Co-DERP1, blue) were analyzed. (B) Phenotype of DO11.10 donor cells in hLN. Statistical analysis is shown in the right panel. n = 5. (C,D) Activation markers CD25 (C) and CD69 (D) on DO11.10 cells in hLN. n = 5. (E) IL-4 expression in DO11.10 cells in hLN. n = 5. (F) IL-10 expression in DO11.10 cells in hLN. n = 5. Data shown represent three independent experiments.