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. 2005 Jan 7;33(1):27–42. doi: 10.1093/nar/gki142

Figure 2.

Figure 2

HPLC chromatograms showing purification of conjugates. (A) Conjugation of Penetratin-Cys with OMe/LNA oligo B activated with Pys (Route B); peaks (i) salts and formamide, (ii) excess Penetratin-Cys (iii) conjugate product, (iv) unconjugated oligoB (Pys). The solid trace is at 280 nm and the dashed trace is at 480 nm, which identifies the fluorescein label on the oligonucleotide. (B) Conjugation of K4Tat-Cys(Pys) + OMe/LNA oligo B (free SH) (Route A); peaks (i) salts and formamide, (ii) excess K4Tat-Cys(Pys), (iii) conjugate product and (iv) unconjugated oligo B (free SH).