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. 2005 Jan 7;33(1):27–42. doi: 10.1093/nar/gki142

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© 2005, the authors Nucleic Acids Research, Vol. 33 No. 1 © Oxford University Press 2005; all rights reserved

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Confocal microscope images of the free uptake of fluorescein-labelled CPP conjugates (green) into HeLa cells (nuclei stained red). (A) Effect of additional equivalents of Tat peptide on uptake of 2.5 μM Tat-Cys-Oligo B, 5 h. (B) Effect of additional equivalents of Penetratin peptide on uptake of 2.5 μM Penetratin-Cys-Oligo B, 5 h. (C) Lipofectamine 2000 delivery of 0.5 μM oligo B, 3 h. Note here the nuclear localization seen (white arrows) in addition to cytosolic uptake.