Table 2.
Properties of conjugates synthesized: retention time on RP–HPLC, calculated and observed masses by MALDI-TOF mass spectrometry, isolated yields and salts used for dissolution of conjugates
| Conjugate | Retention time (min) | Calculated molecular mass | Observed MALDI molecular mass | Isolated yield (%), salts |
|---|---|---|---|---|
| 1 | 10.84 | 6315.22 | 6315.52 | 55, 0 |
| 2 | 11.13 | 6315.22 | 6312.94 | 49, 0 |
| 3 | 12.99 | 6316.25 | 6315.34 | 69, 0 |
| 4 | 13.22 | 6316.25 | 6316.07 | 74, 0 |
| 5 | 13.28 | 7183.31 | 7181.99 | 68, 0 |
| 6 | 13.87 | 7183.31 | 7183.36 | 75, 0 |
| 7 | 12.66 | 6247.15 | 6244.28 | 50, 0 |
| 8 | 12.54 | 6247.15 | 6243.30 | 36, 0 |
| 9 | 15.24 | 7871.20 | 7869.28 | 70, 0 |
| 10 | 18.45 | 8738.26 | 8730.89 | 63, 0.01 M TEAA |
| 11 | 18.04 | 7802.1 | 7801.39 | 40, 0.01 M TEAA |
| 12 | 12.31 | 6540.52 | 6539.62 | 56, 0.1 M TEAA |
| 13 | 15.95 | 7663.92 | 7659.08 | 78, 0 |
| 14 | 9.80 | 6941.00 | 6936.68 | 24, 0.1 M TEAA |
| 15 | 9.94 | 6941.00 | 6944.49 | 55, 0.1 M TEAA |
| 16 | 8.10 | 7792.01 | 7799.79 | 15, 1 M TEAA, 1 M NH4Cl |
| 17 | 8.02 | 7792.01 | 7788.30 | 12, 1 M TEAA |
| 18 | 10.27 | 6828.94 | 6827.88 | 58, 0.1 M TEAA |
| 19 | 8.85 | 8120.43 | 8161.83 (Broad hump) | Not possible to completely dissolve in 2 M TEAA |