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Binding of p4 to each of its binding sites. Increasing amounts of p4 were incubated in 20 μl of binding buffer with a fixed amount of 60 bp fragments (4 nM) containing the 31 bp of the inverted repeats corresponding to site 1, site 2, site 3 or site 4 sequence surrounded by identical but unspecific double-strand DNA. B denotes the protein–DNA complexes and F, free DNA.
