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. 2005 Jan 7;33(1):126–134. doi: 10.1093/nar/gki146

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© 2005, the authors Nucleic Acids Research, Vol. 33 No. 1 © Oxford University Press 2005; all rights reserved

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Binding of p4 to the Mut1 sequence. Increasing amounts of p4 were incubated in 20 μl of binding buffer with a fixed amount of fragment containing the 31 bp of the inverted repeat, corresponding to site 1 or Mut1 sequence surrounded by 31 bp of identical but unspecific double-stranded DNA. B denotes the protein–DNA complexes and F, free DNA. Below, representation of the percentage of p4–DNA complexes formed by increasing concentration of p4 with the wild-type (wt) or mutated site 1 (Mut1).