Table 1.

Oligonucleotides designed for the reconstruction of the sequences required in Figure 5

5HA3A and 4HA3A were the oligonucleotides of the upper strand of region 2 containing five and four hydroxyl radical sets of protections, respectively. Oligonucleotides 3HA3A-site3 and 3HA3A-site4 correspond to the upper strands of region 2 containing the three hydroxyl radical sets of protections included in sites 3 and 4, respectively. 2HA3A was the oligonucleotide for the upper strand of region 2 containing only two of the three hydroxyl radical sets of protections corresponding to site 3. 3HA2cA-site2 and 4HA2cA were the oligonucleotides for the upper strands of region 1 containing the three and four hydroxyl radical sets of protections including site 2. 3HA2cA-site1 and 2HA2cA were the oligonucleotides for the upper strands of region 1 containing the three or only two hydroxyl radical sets of protections corresponding to site 1, respectively. Each oligonucleotide was hybridized with its complementary counterpart to generate the double-stranded sequence used in the p4 binding assays.