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. 2005 Jan 7;33(1):81–94. doi: 10.1093/nar/gki148

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© 2005, the authors Nucleic Acids Research, Vol. 33 No. 1 © Oxford University Press 2005; all rights reserved

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use permissions, please contact journals.permissions@oupjournals.org.

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T_1/2 is dependent on the temperature gradient, not on strand concentration. (A) Normalized melting profiles recorded at 240 nm of the preformed d-(TG₄T)₄ quadruplex (20 μM strand concentration) in 10 mM lithium cacodylate buffer (pH 7.2) with 0.11 M NaCl at four different heating rates (circles: 0.054; crosses: 0.107; squares: 0.26 and triangles 0.4°C/min). (B) T_1/2 of the d-(TG₄T)₄ quadruplex as a function of the average temperature gradient. Note that the ‘true’ equilibrium T_m, which would be obtained with an infinitely slow gradient is calculated to be below 30°C. T_1/2 is provided with a 0.5°C accuracy. (C) Melting and cooling profiles recorded at 295 nm of the preformed d-(TG₄T)₄ quadruplex in 10 mM lithium cacodylate buffer (pH 7.2) with 0.11 M NaCl at two different strand concentrations (20 μM: circles and 200 μM: squares. y-scale values shown on the left and right axis, respectively. 1 cm pathway cuvette for 20 μM, 0.2 cm for 200 μM). Thermal gradient: 0.24°C/min. (D) Dependence of the T_1/2 value (obtained upon heating at a rate of 0.24°C/min) of the preformed d(TG₄T)₄ quadruplex (5–200 μM strand concentration) in 10 mM lithium cacodylate buffer (pH 7.2) with 0.11 M NaCl.