FIG 1.

ASCs that secreted immunogen-specific antibody were present in blood recovered from chinchillas immunized by TCI with a band-aid. Blood was collected 9 days after receipt of the second of two immunizing doses delivered 1 week apart, prior to NTHI challenge. Antibody production by resiquimod- and IL-2-stimulated lymphocytes was assessed by ELISPOT assay. The total numbers of ASCs that produced immunogen-specific IgM, IgG, and IgA were comparable among the cohorts. The mean ± SEM for each antibody isotype within each cohort is presented.