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. 2005 Jan 12;33(1):171–181. doi: 10.1093/nar/gki150

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© 2005, the authors Nucleic Acids Research, Vol. 33 No. 1 © Oxford University Press 2005; all rights reserved

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(A) DNA-dependent DNA polymerase activity of Ty3 DNA polymerase active site mutants Asp213→Glu, Asp214→Asn, Asp214→Glu and Asp214→Gln. Enzyme:substrate ratios and lane notations are as indicated in the legend to Figure 1A. (B and C) DNA-dependent DNA polymerase activity evaluated in the absence (B) and presence of heparin (C), respectively. Lane notations are as in the legend to Figure 1B.