Table 1.

Affinity of Ty3 mutants for duplex DNA

Mutation	Kd (nM)
WT	3.6 ± 0.3
Asp151→Asn151	3.0 ± 1.2
Asp151→Glu151	5.5 ± 0.3
Asp213→Asn213	10.4 ± 1.3
Asp213→Glu213	7.7 ± 2.3
Asp214→Asn214	3.4 ± 1.2
Asp214→Glu214	2.9 ± 1.7
Asp214→Gln214	2.9 ± 1.3

Dissociation constants for each enzyme are the average of duplicate experiments. Values shown ± SD are averaged from two independent experiments.