FIG 7 .

Spatiotemporal chromosome organization of C. glutamicum. Chromosomes are depicted as black lines with oriCs as green circles. In newborn cells, two initial oriCs are located close to the poles. Upon initiation of a new round of replication, sister oriCs segregate nonsynchronously from polar ParB-eYFP clusters and move toward the midcell position, where a new septum is formed. Notably, stages with single chromosomes per cell are absent. (A) Cell cycle of slow-growing cells in MMI medium. A short B period is followed by C and D periods; replication takes place within one generation. (B) Chromosome organization during fast growth in BHI medium. Multifork replication allows for short doubling times (Tds), with a second round of replication starting after the first half of the cell cycle, around 15 min before the previous one terminates.