MUC1 is involved in EAEC-induced PMN migration. (A to C) Monolayers of scrambled control T84 cells or cells with MUC1 shRNA (A and B) or MUC13 shRNA (C) were infected apically with EAEC strains. HBSS+ buffer (-) and the PMN chemoattractant N-formyl-methionyl-leucyl-phenylalanine (fMLP) were included as negative and positive controls, respectively. The data are expressed as the means plus SD for at least triplicate samples, and the data shown are from one representative experiment of at least three independent experiments performed with similar results. ***, P < 0.001; **, P < 0.01; *, P < 0.05.