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. 2017 Jun 6;8(3):e00717-17. doi: 10.1128/mBio.00717-17

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Copyright © 2017 Boll et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 4 — Purified AAFs elicit MUC1-dependent PMN transepithelial migration in the presence of commensal E. coli. (A and C) T84 cell monolayers were infected with live or heat shock-killed commensal E. coli strain MG1655 in the presence or absence (-) of AAF/I or AAF/II, respectively, and assessed for PMN transmigration. (B) T84 monolayers were exposed to beads coated with purified AAF/II or coated with BSA as a negative control and assessed for PMN migration. (D) Monolayers or scrambled control or MUC1 shRNA T84 cells were infected with live E. coli MG1655 in the presence of 10 μg AAF/II and assessed for PMN transmigration. The data are expressed as the means plus SD for at least triplicate samples and represent one of at least three independent experiments performed with similar results. ***, P < 0.001; **, P < 0.01; *, P < 0.05.