FIG 4 .

Viral protein synthesis in complementing and noncomplementing cells. RK/A8A23, RK13, and HeLa cells were infected with 5 PFU/cell of VACV strain WR (WR) or T7/luc for 24 h or with 5 PFU/cell of ΔA23T7/luc vector for 2 or 24 h in the presence or absence of AraC. The cells were lysed, the proteins resolved by SDS polyacrylamide gel electrophoresis, and the viral proteins detected by immunoblotting with antiserum to VACV. GAPDH was detected with a specific antibody as a gel loading control. The masses (in kilodaltons) and positions of marker proteins are indicated on the left.