Sequence alignment and phylogenetic analysis of MdHY5. (a) Protein alignment of MdHY5 and its homologs in Arabidopsis. The zinc-finger domain is boxed. (b) Phylogenetic analysis of MdHY5 and 35 other plants HY5 protein sequences obtained from the NCBI database. MdHY5 is denoted by the red asterisk, and the scale bar indicates the branch length. RsHY5: Raphanus sativus, XP_018445811.1; AtHY5: Arabidopsis thaliana, AT5G11260.1; BrHY5: Brassica rapa, XP_009121971.1; ThHY5: Tarenaya hassleriana, XP_010541629.1; PeHY5: Populus euphratica, XP_011039711.1; MdHY5: Malus domestica, MDP0000586302; PbHY5: Pyrus bretschneideri, XP_009355719.1; FvHY5: Fragaria vesca, XP_004291469.1; ZjHY5: Ziziphus jujuba, XP_015885857.1; PmHY5: Prunus mume, XP_008219477.1; FaHY5: Fragaria ananassa, AKG58815.1; PpHY5: Prunus persica, ONI34365.1; CcHY5: Citrus clementina, XP_006450470.1; BpHY5: Betula platyphylla, AHY20043.1; EgHY5: Eucalyptus grandis, XP_010048982.1; JcHY5: Jatropha curcas, XP_012076602.1; CaHY5: Camptotheca acuminate, APD29065.1; PtHY5: Populus trichocarpa, XP_002308656.1; CmHY5: Cucumis melo, NP_001284656.1; TcHY5: Theobroma cacao, XP_007013841.2; RcHY5: Ricinus communis, XP_002515537.1; VvHY5: Vitis vinifera, XP_010648648.1; MnHY5: Morus notabilis, XP_010110356.1; SiHY5: Sesamum indicum, XP_011081579.1; NnHY5: Nelumbo nucifera, XP_010250037.1; NcHY5: Noccaea caerulescens, JAU18721.1; EsHY5: Eutrema salsugineum, XP_006399627.1; StHY5: Solanum tuberosum, XP_006361723.1; MtHY5: Medicago truncatula, XP_013459310.1; SlHY5: Solanum lycopersicum, NP_001234820.1; PdHY5: Phoenix dactylifera, XP_008785002.1; AcHY5: Ananas comosus, XP_020097860.1; NaHY5: Nicotiana attenuate, XP_019265660.1; DcHY5: Daucus carota, XP_017229054.1; GrHY5: Gentiana rigescens, AIC64080.1; CsHY5: Camelina sativa, XP_010419684.1.