Figure 2.
Effects of light and ABA on the transcript level of MdHY5. (a) Apomictic crabapple (Malus hupehensis) seedlings grown in darkness for 3 days at room temperature were treated with continuous white light for 1, 3, 6, 9 and 12 h. The transcript levels of MdHY5 were determined using RT-PCR (above) and RT-qPCR (below); the value for dark-treated plants was set to 1. (b) Apomictic crabapple (M. hupehensis) seedlings were treated with 50 μM ABA at the indicated time intervals. The expression levels of MdHY5 were monitored by RT-qPCR (above) and RT-PCR (below); the value for untreated plants was set to 1.