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. 2005 Jan 7;33(1):114–125. doi: 10.1093/nar/gki155

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© 2005, the authors Nucleic Acids Research, Vol. 33 No. 1 © Oxford University Press 2005; all rights reserved

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RNase H activity is not observed in the cytoplasm. (A) Polyadenylated preS-Luc mRNA was in vitro transcribed and capped. The hybridization sites for ASO DHBV795 and labeled probes are depicted. The drawing is not to scale (top). Northern-blot analysis upon cotransfection with ASO DHBV 795 is depicted (bottom). Equal amounts of RNA were loaded. Cotransfected renilla mRNA is shown as control. (B) Relative luciferase activity obtained after cotransfection of mRNA and ASO DHBV795. Values are given in percent compared to luciferase activity obtained after cotransfection of control ASO. The number of observations is given in brackets. The asterisk indicates a statistically significant difference.