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. 2005 Jan 7;33(1):143–151. doi: 10.1093/nar/gki156

Figure 2.

Figure 2

Condensation of 3kbDNA, nicked-DNA and a 21mer duplex by hexammine cobalt chloride, as monitored by light scattering. For each data point shown, DNA concentration was 7.5 μM in base pair (5 mM Bis-Tris, 50 μM EDTA, pH 7.0). Light-scattering intensities shown are averages from measurements taken over a 5 min period. Inset: 3kbDNA and nicked-DNA scattering intensities, as a function of hexammine cobalt chloride concentration, normalized to maximum observed intensity. Note: nicked-DNA condensation occurs at a lower hexammine cobalt chloride concentration than 3kbDNA.