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. 2005 Jan 7;33(1):143–151. doi: 10.1093/nar/gki156

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© 2005, the authors Nucleic Acids Research, Vol. 33 No. 1 © Oxford University Press 2005; all rights reserved

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use permissions, please contact journals.permissions@oupjournals.org.

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TEM images of particles formed by various DNA samples upon condensation with the Tat-NLS peptide. (A) Condensates formed by the nicked-DNA duplexes of oligonucleotides N1 and N2. (B) Condensates formed by the gapped-DNA duplexes of oligonucleotides G1 and G2. (C) Condensates formed by the nicked-gapped-DNA duplex of oligonucleotides N1 and G2. (D) Condensates formed by 3kbDNA. (E) Condensates formed by 21mer duplex. For all samples, DNA was 15 μM in base pair, and was condensed by mixing with the Tat-NLS peptide at a charge ratio of 1:2 (DNA phosphate:cationic charged group of the peptide) in 5 mM Bis-Tris, 50 μM EDTA (pH 7.0). Scale bar is 100 nm.