Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 1;8:15692. doi: 10.1038/ncomms15692

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) To scale schematic of a 3D suspended actin intersection encountered by a motor–cargo complex where the approach angle (α) and filament separation (d) are defined. An α of 0° has the centre of the motor–cargo complex vertically above the original filament it is travelling on, which is the same side the intersecting filament is on. d is the centre-to-centre distance between the two intersecting filaments at the point of the intersection. (b) Polar plot predicting whether or not a motor–cargo complex physically interacts with the intersecting filament as a function of d (0–250 nm) and α (0°–360°). To translate this plot into 3D spatial relations between the intersecting filaments and the motor–cargo complex, the illustration of the approach angle (α) in a is used as the point of reference. The originally bound filament on which the motor–cargo complex is travelling on comes in and out of the figure at the graph's origin. The intersecting filament is horizontally in the plane of the figure at a filament separation, d, above the origin with α defined as in a. The magenta line is the predicted spatial boundary at which combinations of d and α determine whether or not the motor–cargo complex can physically interact with the intersecting filament. To scale examples of interaction and non-interaction geometries are illustrated at their respective d and α. The coloured triangles for these examples are identified on the polar plot. (c) Fractional probability plot of motor-complex directional outcomes for experimental (solid bars, n=94) and modelled (slashed bars) data at 3D intersections with predicted interaction geometries. Directional outcomes at 2D experimental intersections (vertical striped bars, n=96) where the motor–cargo complex approached the intersecting filament on the bottom filament. Of the turning outcomes in the 3D experiments, left hand turns occurred 52% of the time with right hand turns the other 48%. For the model the left and right turn probability was equal. The model also predicts (inset) that the intersection directional outcomes are independent of the polarity of the intersecting filament. Error bars are s.d. from six simulations.