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. 2005 Jan 12;33(1):225–234. doi: 10.1093/nar/gki161

Figure 1.

Figure 1

Distinct signals are required for chromatin remodelling at the GM-CSF promoter and GM-CSF gene transcription. (A) Schematic representation of the GM-CSF promoter showing transcription factor binding sites and the CD28RR. DNA fragments amplified by PCR primer sets −I and +I and the HinfI restriction enzyme site are shown. (B) Nuclei from non-stimulated EL-4 T cells (NS) or cells stimulated for 4 h with P, I or P/I as indicated, were incubated with HinfI. Genomic DNA was analysed by real-time PCR using primer set −I. The mean and standard error of three replicate assays are shown. (C and D) Nuclei from cells treated as in (B) were incubated with MNase and genomic DNA was analysed using primer set −I (C) or primer set +I (D). (E) GM-CSF mRNA levels were determined by real-time PCR analysis of cDNA prepared from EL-4 T cells treated as in (B). Data were graphed as a fold change in the mRNA levels compared with NS.