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. 2017 Jun 1;8:15080. doi: 10.1038/ncomms15080

Figure 3. The PTN receptor PTPRZ1 is preferentially expressed by GSCs.

Figure 3

(a) Bivariate correlation analyses showing a positive correlation of PTN and PTPRZ1 expression in human GBMs from the TCGA database (https://tcga-data.nci.nih.gov/tcga). The expressions of PTN and PTPRZ1 in human GBMs were obtained from gene-profiling data from the TCGA database. n=541, P<0.001, Pearson's r test. (b) Expression heatmap of top 10 upregulated genes in GSC lines (n=27) relative to NSTC lines (n=36) from GEO profiles (GEO: GDS3885). Candidate genes enriched in GSCs were identified when the average gene expression ratio of GSCs/NSTCs was ≥3.0-fold. Heatmap was visualized using Cluster/Java Treeview. (c) Immunoblot analysis showing preferential expression of PTPRZ1 and the GSC marker SOX2 in GSCs relative to matched NSTCs derived from human GBMs. α-Tubulin was used for normalization. (d) Immunofluorescence of PTPRZ1 (in green) in GSCs or NSTCs from T0912 GBM. Scale bar represents 50 μm. (e–g) Co-immunofluorescent staining of PTPRZ1 (in green) and the GSC marker SOX2 (in red, e), OLIG2 (in red, f) and CD133 (in red, g) in human GBMs. PTPRZ1 is preferentially expressed in GBM cells expressing GSC markers. Scale bar represents 25 μm. (h–j) Co-immunofluorescent staining of PTPRZ1 (in green) and the GSC marker SOX2 (in red, h), OLIG2 (in red, i) or CD133 (in red, j) in GSC tumourspheres. Co-enrichment of PTPRZ1 with the GSC markers was observed in GSC tumourspheres. Scale bar represents 25 μm. DAPI, 4,6-diamidino-2-phenylindole.