Figure 4. Correlating morphology with in vivo electrophysiology at single-cell level with high efficiency.

(a) Voltage trace of cell-attached recording of a neuron detected at a subpial depth of 768 μm. A threshould of 10% R_p increase was used. (a1) This neuron was spontaneously inactive but voltage fluctuation was observed. (a2) Current injection (100 nA) reversibly evoked spikes. (a3) Electroporation was conducted to deliver the EGFP plasmid into the neuron, increased spiking was observed after electroporation. (b) The neuron recorded was recovered in L6 of V1, showing morphological features of pyramidal neurons, which is consistent with the electrophysiological data. Montage of Z-projection images is shown here, note the pyramidal-shape soma and spiny dendrites. Image stacks were taken from the coronal section with a 63 × , 1.4 NA oil-immersion objective. (c) Experiment yield is high across three mice tested. WM, white matter. Scale bar: 100 μm.