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. 2005 Jan 12;33(1):235–243. doi: 10.1093/nar/gki164

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© 2005, the authors Nucleic Acids Research, Vol. 33 No. 1 © Oxford University Press 2005; all rights reserved

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use permissions, please contact journals.permissions@oupjournals.org.

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Luciferase activity of the pNL_4-3-luciferase (pNL_4-3-luc) fusion gene in COS cells. The cells were co-transfected with the target-expressing plasmid pNL_4-3-luc and either the pL6 plasmid encoding the sgRNAs or pL6-ter serving as a negative control for inhibition using the FuGENE™6 transfection reagent. The plotted data were averaged from three independent experiments, and the bars represent ±SD.