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. 2005 Jan 12;33(1):235–243. doi: 10.1093/nar/gki164

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© 2005, the authors Nucleic Acids Research, Vol. 33 No. 1 © Oxford University Press 2005; all rights reserved

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use permissions, please contact journals.permissions@oupjournals.org.

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Inhibition of HIV-1 gag-p24 product in Jurkat cells expressing stably retrovirus vector-mediated sgRNA. Cells were cultured for 18 days after infection with HIV-1_NL4-3. Small aliquots of supernatant were prepared from each culture on days 1, 3, 6, 9, 12, 15 and 18. HIV-1 gag p24 antigen was determined using CLEIA (Lumipulse) according to the manufacturer's protocol (37): closed diamonds, pLGSN-ΔsgR; closed squares, pLsgRGSN-SL4; and closed triangles, pLsgRGSN-gag-p24.