Fig. 4.

Expression profiling of CM-related genes during the infection process in the normal and infected groups. A, hierarchical clustering of CM-related genes during the infection process. CM genes were divided into three categories: glycolysis, TCA cycle, and pentose-phosphate pathway. B, relative expression levels of selected CM genes at 0 and 72 hpi were validated by qPCR analysis. *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001. The mRNA abundances of aldolase, phosphofructokinase (PFK), enolase, fumarase, fructose-1,6-bisphosphatase (FBP), and glucose-6-phosphate isomerase (GPI) were decreased at 72 hpi, consistent with the RNA-seq data results.