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. 2017 Apr 3;16(6):1081–1097. doi: 10.1074/mcp.M116.066159

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© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 3. — Localization of SUMO1 and SUMO2/3 proteins on diaphragm muscle cross-sections from mechanically ventilated rats. The distribution of SUMOylated proteins changes at different durations of CMV. A and B, left part, cryo-sections stained for NADH-TR, and A and B, right part, immunocytochemical stainings with SUMO1 and SUMO2/3 antibodies in diaphragms from control (ctrl) and mechanically ventilated rats 1 day, 5 days, 10 days, and with BGP-15 for 10 days (10d BGP-15) (means and ±S.D. of SUMO fluorescence intensity per area of 35 oxidative (O, light purple) and 35 glycolytic (G, dark purple) fibers were quantified and plotted (three animals per time point). Scale bars, 50 μm. ***, p < 0.001; **, p < 0.005; *, p < 0.05.