Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 3;16(6):1081–1097. doi: 10.1074/mcp.M116.066159

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

Fig. 4. — Analysis of the endogenous SUMO1- and SUMO2/3-modified proteins in diaphragms of controls and rats exposed to different times of mechanical ventilation and GO comparison between SUMO1 and SUMO2/3 control samples. Preparative gels for the mass spectrometry analysis. A, SUMOylated myofibrillar diaphragm proteins from non-ventilated (control) and from different durations of CMV (1d, 5d, and 10d) without and with BGP-15 (10d BGP-15) were pulled down with monoclonal SUMO1 (21C7 and 76–86); and B, SUMO2 (8A2) antibodies purified from hybridoma cell line, separated by SDS-polyacrylamide gel, stained with Coomassie Blue, cut based on protein molecular weights (dotted lines), and analyzed by high-resolution LC-MS/MS. 1/100 of the total SUMO1 and SUMO2/3 pulldowns samples were probed with anti-SUMO1 (C) and anti-SUMO2/3 (D) antibodies to evaluate the SUMO protein profile and the quality of eluted SUMO immune complexes. Hashtag (#) indicates the SUMOylated RanGAP1 protein. E, Venn diagram comparison between SUMO1 (red circle) and SUMO2 (green circle) identified proteins from control diaphragm. F, Gene Ontology Molecular Function terms were used to find statistical enrichment within the common SUMO1 + SUMO2/3 (blue bars), unique SUMO1 (red bars), and unique SUMO2/3 (green bars) identified on the enriched SUMOylated proteins from not ventilated control muscle diaphragms. All listed terms are significant with −log (p value) >1.30, dashed line. G, classification and comparison using Gene Ontology Cellular Compartments between unique SUMO1 (red bars) and unique SUMO2 (green bars) identified protein targets from control muscle diaphragm compared with the total proteins. The numbers close to the bars indicated the ratios between the unique identified proteins in the cellular compartments and the total SUMO proteins. H, list of six top overlapping pathways; column length indicates the −log (p value) of overlapping p value from Fisher's exact test. Differential yellow line and markers refer to the coverage ratio of identified SUMO1 and SUMO2 proteins in the canonical pathway within the IPA knowledge base. The filling color indicates the predicted activation status of pathways.