Fig. 7.

Modulation of identified SUMO1 and SUMO2/3 muscle proteins during mechanical ventilation. Mechanical ventilation promotes peculiar SUMO post-translational modifications in muscle proteins. Enriched SUMO1 and SUMO2/3 immunocomplexes of control (ctrl) and at different durations of CMV (1d, 5d, 10d, 10d, BGP-15), together with 5 μg of crude control diaphragm lysate (ML) were separated by SDS-polyacrylamide gel and probed with anti-myosin (A), anti-calsequestrin1 (B), anti-triadin (C) and anti-ATPase synthase α-subunit (D). The bands corresponding to the proteins conjugated by SUMO1 are indicated with black arrows, and the protein conjugation by poly-SUMO2/3 is indicated with a square bracket. The white hashtags (#) indicated the low immunoglobulin chains as residues of the immunoprecipitation experiment. Anti-mouse IgG conjugated to Sepharose beads (b) were loaded in parallel to the myosin immunoprecipitants (IPs) as negative control.