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. 2017 Apr 3;16(6):1111–1125. doi: 10.1074/mcp.M117.068130

Fig. 4.

Fig. 4.

Mutations in the N-terminal extension of MOB1A alter binding to upstream MST1 and downstream NDR1 kinases. A, Microscale Thermophoresis binding analysis of NDR112–418 to the indicated MOB1A mutants. Data is plotted as the mean ± S.E. (n = 3). B, Fluorescence polarization binding analysis of the indicated MOB1A mutants to MST1 pT367 (top) and MST pT353 (bottom) peptides. Data is plotted as the mean ± S.E. (n = 3). C, Microscale thermophoresis binding analysis of NDR112–418 to the indicated phosphorylated MOB1A mutants. Data is plotted as the mean ± S.E. (n = 3).