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Description of DiPS. Proteins are subjected to MAAH by suspension in 3 m HCl and microwaving for 4 min. LC-MS/MS amenable peptides are enriched from the hydrolysates by solid-phase extraction and subjected to standard nLC-MS/MS. Resulting spectra are subjected to de novo sequencing using the PEAKS software. The output from PEAKS is used by pTA to extract and assemble peptide tags into consensus contigs.
