Fig. 1.

GFP S65T proteolysis by neprosin under different conditions. Neprosin activity was expressed as percentage of fluorescence lost (100% − % fluorescence recovery) relative to the untreated GFP S65T (without neprosin) under the same reaction condition. The error bars represent standard deviation from at least three replicates. A, effect of protease inhibitors and reducing agents on neprosin activity. B, effect of pH on neprosin activity. The indicated pH is the final pH of the reaction mixture. C, effect of temperature on neprosin activity. ZPP, Z-pro-prolinal inhibitor.